Sperm processing for pre-ICSI analysis
Figure 1.
Epithelial cells in semen sample
Epithelial cells are not normally expected in a sperm sample. They can indicate contamination or reflect how the specimen was collected.
Agarose gel electrophoresis
Figure 2.
TAS2R38 restriction digest (agarose gel)
Purified DNA from the TAS2R38 region after restriction enzyme digest. Lane 1: DNA ladder. Lane 3: individual 1, homozygous wild type (C/C). Lane 5: individual 2, homozygous mutant (T/T). Lane 7: individual 3, heterozygous (C/T). Lane 9: individual 4, homozygous mutant (T/T). Lanes 2, 4, 6, and 8: no-enzyme controls.
See writing for moreSanger sequencing
Figure 3.
TAS2R38 Sanger sequencing
Sanger sequencing data for three individuals in the population, looking at a SNP at position 785 on the gene, TAS2R38. a) homozygous wild type individual; b) homozygous mutant individual; c) heterozygous individual